Volume
7, Number 2, April 2005
Pages 99-100
Hernando Rafael
Letter to the Editor: Secondary Alzheimer started by cryptococcal
meningitis
Page 101 Thomas Ala
Response to the Letter to the Editor
Pages 103-117 P. Hemachandra Reddy,
Geethalakshmi Mani, Byung S. Park, Joline Jacques,
Geoffrey Murdoch, William Whetsell Jr., Jeffrey Kaye, Maria Manczak
Differential loss of synaptic proteins in Alzheimer’s disease:
Implications for synaptic dysfunction
Abstract: The objective of our research was to determine synaptic
protein levels in brain specimens from AD subjects and age-matched control
subjects. Further, to determine whether presynaptic or postsynaptic compartments
of neurons are preferentially affected in AD patients, we studied 3 presynaptic
vesicle proteins (synaptotagmin, synaptophysin, and Rab 3A), 2 synaptic
membrane proteins (Gap 43 and synaptobrevin), and 2 postsynaptic proteins
(neurogranin and synaptopodin) in specimens from AD and age-matched control
brains. Two brain regions – the frontal and parietal cortices –
were assessed for protein levels by immunoblotting analysis. We found
a loss of both presynaptic vesicle proteins and postsynaptic proteins
in all brain specimens from AD patients compared to those from age-matched
control subjects. Further, we found that the loss of synaptic proteins
was more severe in the frontal cortex brain specimens than in the parietal
cortex brain specimens from the AD subjects compared to those from the
control subjects, suggesting that the frontal brain may be critical for
synaptic function in AD. Using immunohistochemistry techniques, we also
determined the distribution pattern of all synaptic proteins in both the
frontal and parietal cortices brain specimens from control subjects. Of
the 7 synaptic proteins studied, the presynaptic proteins synaptophysin
and rab 3A and the postsynaptic protein synaptopodin were the most down-regulated.
Our study suggests that postsynaptic proteins and presynaptic proteins
are important for synaptic function and may be related to cognitive impairments
in AD.
Pages 119-124 Jianping Jia, Erhe
Xu, Yankun Shao, Jianmin Jia, Yongxin Sun, Dan Li (Communicated by Yuan
Luo)
One novel presenilin-1 gene mutation in a Chinese pedigree of
familial Alzheimer’s disease
Abstract: This study is to explore whether there is presenilin
1 (PS 1) gene mutation in Chinese familial Alzheimer’s disease (FAD).
There has been no such systemic research before in China. Using polymerase
chain reaction, single strand conformation polymorphism (PCR-SSCP), followed
by denaturing high performance liquid chromatograph (DHPLC) and DNA sequencing,
we analyzed a Chinese family with early onset AD. The patients in this
family showed a novel missense mutation in exon 4 of the PS1 gene (G to
T change in codon 97), altering valine to leucine acid substitution. Because
the change occurred in conserved domains of this gene, and is not present
in normal controls, this novel mutation is likely to be causative of Chinese
FAD.
Pages 125-133
Jing Zhang, Dave R.
Goodlett, Joseph F. Quinn, Elaine Peskind, Jeffrey A. Kaye, Yong Zhou,
Catherine Pan, Eugene Yi, Jimmy Eng, Qin Wang, Ruedi H. Aebersold, Thomas
J. Montine
Quantitative
proteomics of cerebrospinal fluid from patients with Alzheimer disease
Abstract: Biomarkers to assist in the diagnosis and medical management
of Alzheimer disease (AD) are a pressing need. We have employed a proteomic
approach, microcapillary liquid chromatography mass spectrometry of proteins
labeled with isotope-coded affinity tags (ICAT), to quantify relative
changes in the proteome of human cerebrospinal fluid (CSF) obtained from
the lumbar cistern. Using CSF from well-characterized AD patients and
age-matched controls at 2 different institutions, we quantified protein
concentration ratios of 42% of the 390 CSF proteins that we have identified
and found differences > 20% in over half of them. We confirmed our
findings by western blot and validated this approach by quantifying relative
levels of amyloid precursor protein and cathepsin B in 17 AD patients
and 16 control individuals. Quantitative proteomics of CSF from AD patients
compared to age-matched controls, as well as from other neurodegenerative
diseases, will allow us to generate a roster of proteins that may serve
as specific biomarker panels for AD and other geriatric dementias.
Pages 135-138 Flaubert Tchantchou,
Michael Graves, Eugene Rogers, Daniela Ortiz, Thomas B. Shea
N-acteyl cysteine
alleviates oxidative damage to central nervous system of ApoE-deficient
mice following folate and vitamin E-deficiency
Abstract:
Oxidative stress is an early neurodegenerative insult in Alzheimer’s
disease (AD). Antioxidant mechanisms, including elements of the glutathione
(GSH) pathway, undergo at least a transient compensatory increase that
is apparently insufficient due to continued oxidative damage during disease
progression. Mice deficient in apolipoprotein E, which provide a model
for some aspects of AD, undergo increased oxidative damage to brain tissue
and cognitive decline when maintained on a folate-free diet, despite a
compensatory increase in glutathione synthase transcription and activity
as well as increased levels of GSH. Dietary supplementation with N-acetyl
cysteine (1g/kg diet), a cell-permeant antioxidant and GSH precursor,
alleviated oxidative damage and cognitive decline, and restored glutathione
synthase and GSH levels in ApoE-deficient mice deprived of folate to those
of normal mice maintained in the presence of folate. These data support
the administration of antioxidant precursors to buffer oxidative damage
in neurodegenerative disorders.
Pages 139-148
Xiao-Ping Shi, Katherine
Tugusheva, James E. Bruce, Adam Lucka, Elizabeth Chen-Dodson, Binghua
Hu, Guo-Xin Wu, Eric Price, Robert, B. Register, Janet Lineberger, Ron
Miller, Mei-Jy Tang, Amy Espeseth, Jason Kahana, Abigail Wolfe, Ming-Chih
Crouthamel, Sethu Sankaranarayanan, Adam Simon, Lin Chen, Ming-Tain Lai,
Beth Pietrak, Jillian DiMuzio, Yueming Li, Min Xu, Qian Huang, Victor
Garsky, Mohinder K. Sardana, Daria J. Hazuda (Communicated by Yuan Luo)
Novel mutations introduced at the ß-site of amyloid precursor
protein enhance the production of amyloid ß peptide by BACE1 in
vitro and in cells
Abstract:
Abnormal production and accumulation of amyloid-ß peptide (Aß)
plays a major role in the pathogenesis of Alzheimer’s disease (AD).
ß-secretase (BACE1) is responsible for the cleavage at the ß-site
in amyloid ß protein precursor (AßPP/APP) to generate the
N-terminus of Aß. Here we report the stepwise identification and
characterization of a novel APP-ß-site mutant, "NFEV"
(APP_NFEV) in vitro and in cells. In vitro, the APP_NFEV exhibits 100-fold
enhanced cleavage rate relative to the “wild-type” substrate
(APPwt) and 10-fold increase relative to the Swedish-type mutation variant
(APPsw). In cells, it was preferably cleaved among 24 APP ß-site
mutations tested. More importantly, the APP_NFEV mutant failed to generate
any detectable Aß peptides in BACE1-KO mouse fibroblast cells. The
production of Aß peptides was restored by co-transfecting human
BACE1, demonstrating that BACE1 is the only enzyme responsible for the
processing of APP_NFEV in these cells. Analysis of APP_NFEV cleavage products
secreted in the media revealed that in cells BACE1 cleaves APP_NFEV at
the position between NF and EV, identical to that observed in vitro. A
BACE inhibitor blocked the processing of the APP_NFEV ß-site in
vitro and in cells. Our data indicates that the "NFEV" mutant
is not only an enhanced substrate for BACE1 in vitro, but also a specific
substrate for BACE1 in cells.
Pages 149-157
Johannes Attems, Felix
Lintner, Kurt A. Jellinger
Olfactory Involvement in Aging and Alzheimer’s Disease:
An Autopsy Study
Abstract: Olfactory
dysfunction and tau pathology in the olfactory bulb increase with the
severity of Alzheimer’s disease. We report data of a postmortem
study in the aged. 130 autopsy cases (81 female, 49 male, aged 61-102,
mean 82.48±4.35 SD) years, underwent a standardized neuropathological
assessment with immunohistochemical study of tau pathology in the olfactory
bulb and nerve and of Alzheimer's disease using established criteria including
Braak staging. All cases of definite Alzheimer's disease (Braak stages
5 and 6) (n=40) showed large numbers of neuropil threads and neurofibrillary
tangles, with amyloid deposits in 32.5% and neuritic plaques in one single
case in the olfactory system. Braak stage 4 (n=27) was associated with
mild to moderate tau pathology in 85.2%, and amyloid plaques in 1.1%,
Braak stage 3 (n=28) with olfactory tau lesions in 37.0% and amyloid deposits
in one single case, Braak stages 3 and 4 with olfactory tau lesions in
61.1%. Braak stage 2 (n=15) showed olfactory tau pathology in 31.2%, whereas
Braak stages 0 and 1 (n=15) were all negative. The olfactory system tau
score showed highly significant correlations with neuritic Braak stages
in the brain, while both scores showed significant but low correlations
with age. These data confirm previous studies demonstrating abundant tau
pathology in the olfactory system in all definite Alzheimer’s disease
cases, in two-thirds of limbic Alzheimer’s disease, and in almost
one-third of non-demented elderly persons with Braak stage 2. There are
strong correlations between tau pathology in the olfactory and limbic
systems, both with similar increase in severity. Clinical dementia correlated
with both Braak and olfactory system tau scores. Since the involvement
of both systems is associated with a high risk of cognitive decline, future
studies should validate the sensitivity of olfactory mucosa biopsies in
the diagnosis of Alzheimer’s disease.
Pages 159-171 Syed I.A. Zaidi, Sandra
L. Richardson, Sabina Capellari, Li Song, Bernardino Ghetti, Man-Sun Sy,
Pierluigi Gambetti, Robert B. Petersen
Characterization of the F198S Prion Protein Mutation: Enhanced
Glycosylation and Defective Refolding
Abstract: Prion diseases are associated with the accumulation of a misfolded,
protease resistant form of the prion protein, PrPres. In humans there
are a variety of different prion related diseases that are sporadic, inherited,
or acquired by infection. Gerstmann-Straussler-Sheinker syndrome (GSS)
is an inherited prion disease in which PrPres accumulates as amorphous
aggregates as well as in amyloid plaques. GSS has been associated with
a variety of point mutations in the prion protein: 102, 105, 117, 131,
145, 187, 198, 202, 212, 217, and 232. The F198S mutation was discovered
in a large Indiana kindred. Previous studies in vitro have shown that
the 198 mutation results in structural instability of the prion protein.
In the current study, we demonstrate in a cell model that the F198S mutant
protein can be folded properly in a cellular context, but is unable to
refold to a native state after denaturation. Further, the F198S mutation
significantly affects glycosylation of the mutant protein.
Pages 173-180 Transcript: Alzheimer
Research Forum Live Discussion
Brain Derived Neurotrophic Factor and Alzheimer's Disease—What
Is the Connection?
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