%0 Journal Article %J J Alzheimers Dis %D 2016 %T Discrepancy in Expression of β-Secretase and Amyloid-β Protein Precursor in Alzheimer-Related Genes in the Rat Medial Temporal Lobe Cortex Following Transient Global Brain Ischemia. %A Pluta, Ryszard %A Kocki, Janusz %A Ułamek-Kozioł, Marzena %A Petniak, Alicja %A Gil-Kulik, Paulina %A Januszewski, Sławomir %A Bogucki, Jacek %A Jabłoński, Mirosław %A Brzozowska, Judyta %A Furmaga-Jabłońska, Wanda %A Bogucka-Kocka, Anna %A Czuczwar, Stanisław J %K Amyloid beta-Protein Precursor %K Amyloid Precursor Protein Secretases %K Animals %K Brain Ischemia %K Disease Models, Animal %K Down-Regulation %K Female %K Rats %K Rats, Wistar %K Temporal Lobe %K Time Factors %X

Brain ischemia may be causally related with Alzheimer's disease. Presumably, β-secretase and amyloid-β protein precursor gene expression changes may be associated with Alzheimer's disease neuropathology. Consequently, we have examined quantitative changes in both β-secretase and amyloid-β protein precursor genes in the medial temporal lobe cortex with the use of quantitative rtPCR analysis following 10-min global brain ischemia in rats with survival of 2, 7, and 30 days. The greatest significant overexpression of β-secretase gene was noted on the 2nd day, while on days 7-30 the expression of this gene was only modestly downregulated. Amyloid-β protein precursor gene was downregulated on the 2nd day, but on days 7-30 postischemia, there was a significant reverse tendency. Thus, the demonstrated alterations indicate that the considerable changes of expression of β-secretase and amyloid-β protein precursor genes may be connected with a response of neurons in medial temporal lobe cortex to transient global brain ischemia. Finally, the ischemia-induced gene changes may play a key role in a late and slow onset of Alzheimer-type pathology.

%B J Alzheimers Dis %V 51 %P 1023-31 %8 2016 %G eng %N 4 %1 http://www.ncbi.nlm.nih.gov/pubmed/26890784?dopt=Abstract %R 10.3233/JAD-151102