%0 Journal Article %J J Alzheimers Dis %D 2023 %T Accumulation of pTau231 at the Postsynaptic Density in Early Alzheimer's Disease. %A Lilek, Jaclyn %A Ajroud, Kaouther %A Feldman, Alexander Z %A Krishnamachari, Sesha %A Ghourchian, Shadi %A Gefen, Tamar %A Spencer, Callen L %A Kawles, Allegra %A Mao, Qinwen %A Tranovich, Jessica F %A Jack, Clifford R %A Mesulam, M-Marsel %A Reichard, R Ross %A Zhang, Hui %A Murray, Melissa E %A Knopman, David %A Dickson, Dennis W %A Petersen, Ronald C %A Smith, Benjamin %A Ashe, Karen H %A Mielke, Michelle M %A Nelson, Kathryn M %A Flanagan, Margaret E %B J Alzheimers Dis %V 92 %P 241-260 %8 2023 Mar 07 %G eng %N 1 %1 https://www.ncbi.nlm.nih.gov/pubmed/36744338?dopt=Abstract %R 10.3233/JAD-220848 %0 Journal Article %J J Alzheimers Dis %D 2023 %T Neuropathology of Anti-Amyloid-β Immunotherapy: A Case Report. %A Castellani, Rudolph J %A Shanes, Elisheva %A McCord, Matthew %A Reish, Nicholas J %A Flanagan, Margaret E %A Mesulam, M-Marsel %A Jamshidi, Pouya %X

Host responses to anti-amyloid-β (Aβ) antibody therapy are evident in neuroimaging changes and clinical symptoms in a subset of clinical trial subjects receiving such therapy. The pathological basis for the imaging changes and clinical symptoms is not known, nor is the precise mechanism of Aβ clearing. We report the autopsy findings in a 65-year-old woman who received three open label infusions of the experimental anti-Aβ drug lecanemab over about one month. Four days after the last infusion, she was treated with tissue plasminogen activator for acute stroke symptoms and died several days later with multifocal hemorrhage. Neuropathological examination demonstrated histiocytic vasculitis involving blood vessels with cerebral amyloid angiopathy. Fragmentation and phagocytosis of vascular Aβ were present throughout the cerebral cortex. Phagocytosis of parenchymal Aβ plaques was noted. Changes suggestive of Aβ and phosphorylated tau "clearing" were also noted. The findings overall suggest that anti-Aβ treatment stimulated a host response to Aβ, i.e., target engagement. The findings also provide evidence that amyloid-related imaging abnormalities might be indicative of an Aβ phagocytic syndrome within cerebral vasculature and parenchymal brain tissue in some cases.

%B J Alzheimers Dis %V 93 %P 803-813 %8 2023 May 16 %G eng %N 2 %R 10.3233/JAD-221305 %0 Journal Article %J J Alzheimers Dis %D 2016 %T Postmortem Adult Human Microglia Proliferate in Culture to High Passage and Maintain Their Response to Amyloid-β. %A Guo, Ling %A Rezvanian, Aras %A Kukreja, Lokesh %A Hoveydai, Ramez %A Bigio, Eileen H %A Mesulam, M-Marsel %A El Khoury, Joseph %A Geula, Changiz %X

Microglia are immune cells of the brain that display a range of functions. Most of our knowledge about microglia biology and function is based on cells from the rodent brain. Species variation in the complexity of the brain and differences in microglia response in the primate when compared with the rodent, require use of adult human microglia in studies of microglia biology. While methods exist for isolation of microglia from postmortem human brains, none allow culturing cells to high passage. Thus cells from the same case could not be used in parallel studies and multiple conditions. Here we report a method, which includes use of growth factors such as granulocyte macrophage colony stimulating factor, for successful culturing of adult human microglia from postmortem human brains up to 28 passages without significant loss of proliferation. Such cultures maintained their phenotype, including uptake of the scavenger receptor ligand acetylated low density lipoprotein and response to the amyloid-β peptide, and were used to extend in vivo studies in the primate brain demonstrating that inhibition of microglia activation protects neurons from amyloid-β toxicity. Significantly, microglia cultured from brains with pathologically confirmed Alzheimer's disease displayed the same characteristics as microglia cultured from normal aged brains. The method described here provides the scientific community with a new and reliable tool for mechanistic studies of human microglia function in health from childhood to old age, and in disease, enhancing the relevance of the findings to the human brain and neurodegenerative conditions.

%B J Alzheimers Dis %V 54 %P 1157-1167 %8 2016 Oct 04 %G eng %N 3 %1 http://www.ncbi.nlm.nih.gov/pubmed/27567845?dopt=Abstract %R 10.3233/JAD-160394