Title | Increased Albumin Oxidation in Cerebrospinal Fluid and Plasma from Alzheimer's Disease Patients. |
Publication Type | Journal Article |
Year of Publication | 2018 |
Authors | Costa, M, Horrillo, R, Ortiz, AMaría, Pérez, A, Mestre, A, Ruiz, A, Boada, M, Grancha, S |
Journal | J Alzheimers Dis |
Volume | 63 |
Issue | 4 |
Pagination | 1395-1404 |
Date Published | 2018 |
ISSN | 1875-8908 |
Abstract | BACKGROUND: Oxidative stress in the brain and peripheral systems is considered a major player in Alzheimer's disease (AD). Albumin is the main transporter and the main extracellular antioxidant in the human body. OBJECTIVE: Here we explore for the first time the oxidation status of cerebrospinal fluid (CSF) and plasma albumin in AD in comparison to healthy subjects. METHODS: Plasma and CSF samples were obtained from mild-moderate AD patients and control healthy age-matched donors. Albumin redox state forms (reduced: HMA; reversibly oxidized: HNA1; irreversibly oxidized: HNA2) were determined by HPLC. Albumin post-translational modifications (PTM) analysis was performed by mass spectrometry. RESULTS: HPLC showed less HMA in AD plasma than in controls (54.1% versus 65.2% ; p CONCLUSION: CSF albumin oxidation in AD patients was dramatically increased comparing to healthy controls, while in plasma this increase was smaller. CSF albumin in AD patients was much more oxidized than in plasma, but this effect was not observed in healthy controls. These results suggest that albumin oxidation, especially in CSF, and its role in AD deserves further investigation. |
DOI | 10.3233/JAD-180243 |
Alternate Journal | J. Alzheimers Dis. |
PubMed ID | 29782326 |
PubMed Central ID | PMC6004933 |