Title | Effect of Tween-20 on Core Biomarkers Measured in Cerebrospinal Fluid from Patients with Alzheimer's Disease, Mild Cognitive Impairment, or Healthy Control Individuals. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Berge, G, Lauridsen, C, Sando, SBotne, Holder, DJoseph, Møller, I, Aasly, JOlav, Bråthen, G, Savage, MJosephine, White, LRosemary |
Journal | J Alzheimers Dis |
Volume | 49 |
Issue | 2 |
Pagination | 493-502 |
Date Published | 2016 |
ISSN | 1875-8908 |
Keywords | Aged, Alzheimer Disease, Amyloid beta-Peptides, Cognitive Dysfunction, Enzyme-Linked Immunosorbent Assay, Female, Humans, Longitudinal Studies, Male, Middle Aged, Peptide Fragments, Phosphorylation, Polysorbates, ROC Curve, Surface-Active Agents, tau Proteins |
Abstract | BACKGROUND: There is substantial variation caused by preanalytical procedures in the measurement of cerebrospinal fluid (CSF) biomarkers for Alzheimer's disease (AD) reported in the literature. OBJECTIVE: Determine whether the detergent Tween-20 improves diagnostic accuracy. METHODS: CSF proteins (Aβ42, Aβ40, total tau, and phosphorylated tau) were measured by standard ELISA, in uncentrifuged CSF with or without 0.05% Tween-20 from patients with AD or amnestic mild cognitive impairment, and healthy elderly controls. In the main study, collection tubes containing Tween-20 (Sarstedt 15 mL) were filled with 5 mL CSF to ensure consistent detergent concentration across subsequent aliquots into Corning 2 mL tubes. These latter were also the primary collection vessel for samples without Tween-20. The effect of centrifugation, and extra tube transfer of samples with Tween-20 were also examined. RESULTS: 0.05% Tween-20 significantly increased mean measured CSF concentration of Aβ42 (30% ), Aβ40 (23% ), and total tau (4% ), but not phosphorylated tau. Generally, these increases were similar in all groups, although for Aβ42, the mean percentage increase with Tween-20 was slightly larger for AD. Areas under receiver-operator characteristic curves were similar whether Tween-20 was present or not. Centrifuged CSF without Tween-20 significantly reduced the measured concentration of Aβ42 versus non-centrifuged samples, a difference not seen when detergent was added. Similar CSF Aβ42 levels were found whether Tween-20 was added at collection in an extra tube or directly to the main collection tube. CONCLUSION: Addition of Tween-20 to CSF did not improve differentiation of patients from controls. |
DOI | 10.3233/JAD-150234 |
Alternate Journal | J. Alzheimers Dis. |
PubMed ID | 26484901 |