Title | Discrepancy in Expression of β-Secretase and Amyloid-β Protein Precursor in Alzheimer-Related Genes in the Rat Medial Temporal Lobe Cortex Following Transient Global Brain Ischemia. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Pluta, R, Kocki, J, Ułamek-Kozioł, M, Petniak, A, Gil-Kulik, P, Januszewski, S, Bogucki, J, Jabłoński, M, Brzozowska, J, Furmaga-Jabłońska, W, Bogucka-Kocka, A, Czuczwar, SJ |
Journal | J Alzheimers Dis |
Volume | 51 |
Issue | 4 |
Pagination | 1023-31 |
Date Published | 2016 |
ISSN | 1875-8908 |
Keywords | Amyloid beta-Protein Precursor, Amyloid Precursor Protein Secretases, Animals, Brain Ischemia, Disease Models, Animal, Down-Regulation, Female, Rats, Rats, Wistar, Temporal Lobe, Time Factors |
Abstract | Brain ischemia may be causally related with Alzheimer's disease. Presumably, β-secretase and amyloid-β protein precursor gene expression changes may be associated with Alzheimer's disease neuropathology. Consequently, we have examined quantitative changes in both β-secretase and amyloid-β protein precursor genes in the medial temporal lobe cortex with the use of quantitative rtPCR analysis following 10-min global brain ischemia in rats with survival of 2, 7, and 30 days. The greatest significant overexpression of β-secretase gene was noted on the 2nd day, while on days 7-30 the expression of this gene was only modestly downregulated. Amyloid-β protein precursor gene was downregulated on the 2nd day, but on days 7-30 postischemia, there was a significant reverse tendency. Thus, the demonstrated alterations indicate that the considerable changes of expression of β-secretase and amyloid-β protein precursor genes may be connected with a response of neurons in medial temporal lobe cortex to transient global brain ischemia. Finally, the ischemia-induced gene changes may play a key role in a late and slow onset of Alzheimer-type pathology. |
DOI | 10.3233/JAD-151102 |
Alternate Journal | J. Alzheimers Dis. |
PubMed ID | 26890784 |